Tg(MMTV-tTA,PyVT-tetO-cre)6Atai

One transgenic vector consisted of cDNA encoding the tetracycline transactivator tTA (containing the tet repressor, TetR and the transcriptional activation domain of protein 16 of the herpes simplex virus, VP16) downstream of the MMTV-LTR promoter. The tTA fragment is flanked by the acceptor splice site and the polyadenylation site from the rabbit beta-globin gene. A second transgene was composed of the polyomavirus middle t antigen (mT; PyVT) cDNA upstream of a bidirectional tetracycline responsive element (with heptamerized tet operator sequences - TetR binding sites flanked by CMV promoters). The cre sequence was inserted after tetO between an acceptor splice site and polyadenylation site. In this construct, simulataneous expression of both cre and PyVT is inducible. The 2 purified constructs were coinjected into fertilized C57BL/6N oocytes. Four founders were generated with line 6 transmitting the transgenes over many generations. (J:168057)