Using the MICER gene targeting system, 5'Hprt and 3'Hprt vectors containing exons from either side of the targeted region, single loxP sites, and a neomycin or puromycin drug selection cassette were used to flank the region. Cre-mediated recombination removed Eif2c3, Eif2c1, and Eif2c4. (J:101977)