A loxP site was inserted upstream of exon 3, and an FRT-flanked neo cassette with a 5' loxP site was inserted dowstream of exon 3. Flp-mediated recombination removed the neo cassette leaving exon 3 floxed. (J:166984)
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A loxP site was inserted upstream of exon 3, and an FRT-flanked neo cassette with a 5' loxP site was inserted dowstream of exon 3. Flp-mediated recombination removed the neo cassette leaving exon 3 floxed. (J:166984)