Tow loxP sites were inserted to flank part of intron 1, exons 2-4 and the 3'UTR. An FRT site-flanked neomycin resistance gene was introduced followed by the second loxP site, and removed later by Flp-mediated recombination. A beta-galactosidase gene was also inserted after the second loxP site. Cre-mediated recombination of the loxP-flanked gene segment creates an in frame translation of beta-galactosidase. (J:167000)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count