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An enhanced yellow fluorescence protein (eYFP) cassette was introduced in frame with the first ATG in exon 1, followed by a neo cassette flanked by two Frt sites. The neo cassette was removed by Flp-mediated recombination, resulting in a continuous reading frame from the initiating codon, through eYFP and the residual FRT to the second amino acid. No yellow fluorescent signal was detected in any tissue. Real-time PCR and Western blot analysis showed decreased expression in ovaries from homozygous females. (J:166778)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
