A loxP site was inserted upstream of exon 2 and an FRT flanked neo cassette and second loxP site were inserted downstream of exon 2 via homologous recombination. Flp mediated recombination removed the neo cassette leaving exon 2 floxed. (J:166721)
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A loxP site was inserted upstream of exon 2 and an FRT flanked neo cassette and second loxP site were inserted downstream of exon 2 via homologous recombination. Flp mediated recombination removed the neo cassette leaving exon 2 floxed. (J:166721)