A loxP site was inserted at the end of intron 4 and a neo cassette flanked by two loxP sites were inserted into the middle of intron 6. The neo cassette was deleted by transiently transfection of an expression vector of Cre recombinase in the ES cells. Two independent heterozygous ES cell clones. (J:166211)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count