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A targeting construct containing the eGFPCreERT2 (GCE) cassette and FRT site flanked Kanamycin/neomycin selection cassette was inserted into the consensus start ATG codon of the gene in a BAC (RP23-213P2). The PGK-Neo/Kan cassette was removed by transient Flpe-recombinase expression. The resulting BAC transgene was microinjected into (C57BL/6 x DBA)F1 donor eggs. Founder line 22 was subsequently established. GFP fluorescence is not detectable by fluorescent microscope examination of whole embryos, bones or neural tube sagittal slices from embryos aged 15.5dpc. Tamoxifen induced cre recombinase activity is detected in a subset of the GFP immunoreactive-positive cells in bone. (J:159099)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
