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A targeting construct containing the eGFPCreERT2 (GCE) cassette and Frt site flanked Kanamycin/neomycin selection cassette was inserted into the consensus start ATG codon of the gene in a BAC (RP23-183L17). The PGK-Neo/Kan cassette was removed by transient Flpe-recombinase expression. The resulting BAC transgene was microinjected into (C57BL/6 x DBA)F1 donor eggs. Founder line 30 was subsequently established. Native GFP expression is detectable in the urogenital system of E15.5 embryos. Transgenic cre expression is detected in developing nephrons of the kidney, Wolfian and Mullerian ducts, and vasculature of the urogenital system of hemizygous 15.5 dpc embryos. Possible cre recombinase activity is detected in small renal arteries. (J:159099)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
