A loxP site was inserted upstream of exon 3, and an FRT and loxP flanked neo cassette was inserted downstream of exon 4. Flp mediated recombination removed the neo cassette leaving exons 3 and 4 floxed. (J:166421)
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A loxP site was inserted upstream of exon 3, and an FRT and loxP flanked neo cassette was inserted downstream of exon 4. Flp mediated recombination removed the neo cassette leaving exons 3 and 4 floxed. (J:166421)