A loxP site was inserted upstream of exon 11 and an FRT-flanked neo cassette with a 5' loxP site was inserted downstream of exon 13. Flp-mediated recombination removed the neo cassette leaving exons 11 through 13 floxed. (J:165114)
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A loxP site was inserted upstream of exon 11 and an FRT-flanked neo cassette with a 5' loxP site was inserted downstream of exon 13. Flp-mediated recombination removed the neo cassette leaving exons 11 through 13 floxed. (J:165114)