Exon 2 was floxed and a FRT flanked neomycin cassette was inserted within intron 2 by homologous recombination. The selection cassette and exon 2 were removed by flpe and cre mediated recombination respectivly. (J:164205)
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Exon 2 was floxed and a FRT flanked neomycin cassette was inserted within intron 2 by homologous recombination. The selection cassette and exon 2 were removed by flpe and cre mediated recombination respectivly. (J:164205)