Tg(GATA3-EGFP)1Smal

A BAC containing the GATA3 locus was engineered so that the first exon of the gene was replaced with the coding sequence for EGFP. An frt-flanked kanamycin resistance gene driven by the prokaryotic EM7 promoter was used for selection of correctly recombined colonies. The selection cassette was subsequently removed by flp recombinase in bacteria. The 180 kb final BAC was injected into pronuclei, generating four founder lines. One line was selcected for further analysis. No line numbers were specified in the reference. (J:164670)