Pdpk1^tm1.1Daca

Exon 4 was replaced with one in which nucleotide substitution resulted in the amino acid substitution of alanine for lysine at position 111 (K111A) and a neo cassette. Cre mediated recombination removed the neo cassette. The absence of catalytic activity was confirmed in E11.5 to E12.5 embryo lysates despite confirmed protein expression. (J:165626)