A loxP site was inserted up stream of exon 2 and an FRT flanked neo/kan cassette and second loxP site were inserted downstream of exon 2 via homologous recombination. Flp mediated recombination removed the neo/kan cassette. (J:164582)
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A loxP site was inserted up stream of exon 2 and an FRT flanked neo/kan cassette and second loxP site were inserted downstream of exon 2 via homologous recombination. Flp mediated recombination removed the neo/kan cassette. (J:164582)