Hprt1^{tm43(Ple140-lacZ)Ems}

The Ple140-lacZ transgene (pEMS1612) was designed with the 4379 bp Ple140 minipromoter (NR2E1-F; derived from a portion of the promoter of the NR2E1 gene and five upstream putative regulatory elements from the Orphan nuclear receptor NR2E1 (NR2E1) gene) upstream of a frt-flanked beta-galactosidase (lacZ) gene, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprtb-m3 mutant locus on the X chromosome. (J:144244, J:164356)