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A vector containing a cre-loxP-neo-loxP cassette flanked by two short homologous sequences was electroporated into the bacteria (EL250) together with a modified BAC (RP23-16G10) containing the Mcpt8 locus. By homologous recombination, the coding sequence of cre recombinase was inserted directly behind the Mcpt8 start codon. The neo cassette was deleted by growing bacteria in arabinose-containing medium and the BAC clone was injected into the pro-nucleus of fertilized C57BL/6 oocytes. The copy number of the integrated BAC in two different founder lines was determined by quantitative PCR for Mcpt8 (5 copies in line #1 and 7 copies in line #2.) (J:164432)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
