Gene editing
Del(7Gabrb3-Ube3a)1Yhj
Alias:
No other aliases for this model
Basic Information
Exon2 of Ube3a was replaced with a cassette containing Hprt exons1-2, a loxP site, and a neomycin selection cassette. On the same chromosome, part of Gabrb3 exon 1 and all of exon 2 were replaced by a cassette containing Hprt exons 3-9, another loxP site and a puromycin selection cassette. Properly targeted ES cells were then subjected to transient cre expression. Excision of sequence between Gabrb3 and Ube3a resulted in the creation of a complete Hprt gene that was then used to select for the desired deletion event. The deleted interval extended from 64846987 bp to 66527319 bp) based on the NCBI Build 37 assembly and includes Atp10a. (J:164010)
Basic Information
MGI ID
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Because of their genetic and physiological similarities with humans, mouse models are essential for understanding human illnesses. Advances in molecular biology and gene editing improve disease replication in mice, allowing researchers to get a better understanding of disease causes, identify biomarkers, and create treatments.
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#) :related diseases count
Phenotypes：
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References
Disease models are key instruments in biomedical research, and their literature reports are critical to the advancement of research. These publications provide researchers with a comprehensive theoretical foundation by detailing the design procedures, application regions, and outcomes analysis of disease models.
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BioGPT