Using BAC RP23-63G7 exon 9 was floxed and a FRT flanked neo cassette was inserted within intron 9, 5' to the second loxP site. The selection cassette was removed by flp mediated recombination. (J:163751)
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Using BAC RP23-63G7 exon 9 was floxed and a FRT flanked neo cassette was inserted within intron 9, 5' to the second loxP site. The selection cassette was removed by flp mediated recombination. (J:163751)