Tg(Thy1-cre/ERT2,-EYFP)HGfng

The transgene was designed with a creERT2 fusion gene, two copies of the mouse Thy1 promoter, and an enhanced yellow fluorescent protein (EYFP) cDNA sequence. The creERT2 fusion gene (cre/ERT2) is cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand-binding domain. Each Thy1 promoter region is a modified regulatory region of the murine thy1.2 gene (extending from the promoter to the intron following exon 4, excluding exon 3 and its flanking introns). The two copies are placed "back to back" to function bidirectionally; one copy drives expression of CreERT2, the other copy drives expression of EYFP. Founder line H (SLICK-H) was subsequently established and backcrossed to C57BL/6J for 4 generations. (J:134019)