Ccn2^tm1.1Vlcg

A conditional by inversion (COIN) intron consisting of a lox66_SA-Egpf-polyA_lox71 was inserted in the antisense orientation into exon 2 via homologous recombination, splitting exon 2 into 2 new exons (120 and 103 bp in length). The two lox sites are in reverse complementation orientation, thus cre mediated recombination will irreversibly invert the inserted sequence. In addition, an FRT flanked hygromycin-delta-thymidine kinase cassette was inserted after the COIN intron. Flp mediated recombination removed the hygromycin cassette. Expression analysis confirmed expression of full length mRNA in homozygous mice. (J:163027)