The coding region of exon 1 through exon 8 was replaced with (5' to 3'): a loxP site, GFP, lox511 site, and FRT flanked neo cassette. The absence of transcript expression was confirmed by RT-PCR on testes extracts. (J:162590)
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The coding region of exon 1 through exon 8 was replaced with (5' to 3'): a loxP site, GFP, lox511 site, and FRT flanked neo cassette. The absence of transcript expression was confirmed by RT-PCR on testes extracts. (J:162590)