The GFP coding sequence was inserted into the pCAGGs expression vector. The construct was linearized and injected into (C57BL/6 x C3H)F1 fertilized eggs and transferred into pseudopregnant ICR females. Three lines were produced. Lines #2 and #14 carried about 5 copies of the transgene and #10 had about 50 copies. Lines 2 and 10 were studied further. (J:162225)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
(C57BL/6 x C3H)F1
--
Insertion
--
--
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6

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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