One loxP site was inserted upstream of exon 3 and a neomycin selection cassette flanked by tandemly arranged loxP and FRT sites was placed downstream. Transient cre expression was then used to create ES cells in which exon 3 was floxed. (J:162048)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count