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This transgene contains the tet operon (tetO) and a minimal CMV promoter controlling expression of a cDNA encoding a dominant-negative mutant rat cAMP-responsive element binding protein 1 (CREB1), alpha isoform, bearing a FLAG peptide tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys). The coding sequence is followed by the SV40 intron and polyadenylation signal. The mutant CREB1 has alanine in place of the critical regulatory serine at position 133, eliminating the phosphorylation site. Unphosphorylated CREB binds to cAMP response elements (CREs), blocking access by wild-type CRE and other CRE-binding proteins. (J:128618)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
