A loxP site was inserted upstream of exon 17, and an frt flanked neo cassette with a 3' loxP site was inserted downstream of exon 18. The frt sites were mutated and could not be used to remove the neo cassette, which remains intact. (J:160568)
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A loxP site was inserted upstream of exon 17, and an frt flanked neo cassette with a 3' loxP site was inserted downstream of exon 18. The frt sites were mutated and could not be used to remove the neo cassette, which remains intact. (J:160568)