A loxP site was inserted upstream of exon 3 and a floxed PGK-neomycin selection cassette was inserted downstream of the same exon. The PGK-neo cassette was then exised from propeerly targeted ES cells leaving exon 3 floxed. (J:124962)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count