A loxP site was inserted upstream of exon 3 and a floxed PGK-neomycin selection cassette was inserted downstream of the same exon. The PGK-neo cassette was then exised from propeerly targeted ES cells leaving exon 3 floxed. (J:124962)
查看原文 参与反馈
A loxP site was inserted upstream of exon 3 and a floxed PGK-neomycin selection cassette was inserted downstream of the same exon. The PGK-neo cassette was then exised from propeerly targeted ES cells leaving exon 3 floxed. (J:124962)