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Two loxP sites were introduced to flank exon 1 by recombineering. An engineered FRT-Neo-FRT selection cassette was immediately preceding the second loxP site. The first exon was deleted by Cre-mediated recombination. RT-PCR and immunostaining confirmed the absence of mRNA and protein expression in the testis of homozygous mice. Western blots performed on lysates of mouse lung, kidney, heart, spleen, and testis confirmed the loss of the protein expression. (J:160452)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
