A loxP site was inserted upstream of exon 20 and an frt flanked neo cassette with a 3' loxP site was inserted downstream of exon 20. Flp mediated recombination removed the neo cassette leaving exon 20 floxed. (J:161124)
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A loxP site was inserted upstream of exon 20 and an frt flanked neo cassette with a 3' loxP site was inserted downstream of exon 20. Flp mediated recombination removed the neo cassette leaving exon 20 floxed. (J:161124)