A targeting vector containing an FRT site flanked NEO cassette was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 3 of the targeted gene, and loxP sites were placed flanking exon 3. Flp mediated recombination removed the neo cassette leaving exon 3 floxed. (J:160807)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count