A loxP site was inserted in intron 2 and a floxed neo cassette was inserted in intron 8 via homologous recombination. Cre mediated recombination removed the neo cassette leaving exons 3 - 8 floxed. (J:123288)
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A loxP site was inserted in intron 2 and a floxed neo cassette was inserted in intron 8 via homologous recombination. Cre mediated recombination removed the neo cassette leaving exons 3 - 8 floxed. (J:123288)