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A BAC containing the Runx2 locus was modified through ET recombination to insert a codon-improved cre recombinase sequence and an ampicillin resistance gene flanked by two FRT sites at the translational start site of the distal promoter (P1) of the Runx2 gene. Subsequently in the recombinant BAC, the ampicillin resistance cassette was removed by arabinose-induced FLP recombination.The linearized construct was injected into the pronuclei of FVB/N oocytes. Three founder lines were established and one was chosen for experimental use based on its expression pattern. (J:160669)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
