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A modified E14 ES cell line heterozygous for the Slc22a1tm1Ahs allele was targeted a second time. Exon 1 (encoding the start codon, the putative TMD1 domain, and extracellular loop between the putative TMD1 and TMD2 domains), as well as upstream sequence were replaced with a neomycin selection cassette inserted by homologous recombination. Absence of Slc22a2 mRNA in double homozygous mutant mice was confirmed by Northern blotting and RNase protection analysis. Western analysis verified that no Slc22a1 protein was detectable in livers and kidneys of double mutant mice. (J:86270)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
