Cldn16^tm1.1Dmu

An FRT/loxP-flanked pgk-neo cassette and an additional loxP sequence were inserted to flank exon 2, which allowed subsequent deletion of the exon as well as the neomycin resistance cassette. Elimination of exon 2 leads to a frameshift and early stop codon and truncation of the protein. The deletion of exon 2 as well as the neomycin resistance gene was accomplished via Cre-mediated recombination. Western blot analysis showed the absence of protein expression in kidney tissue from homozygous mice. (J:159706)