Acacb^tm1.1Lowl

LoxP sites were positioned upstream of the preceding exon and downstream of the biotin binding domain exon, with an Frt flanked kanamycin resistance cassette inserted before the 3' loxP site. The Frt-flanked selection cassette was removed by Flpe recombinase-mediated recombination. Splicing of the remaining exons following Cre-mediated deletion would introduce a frameshift and nonsense mutation after Asp865 in any translated protein. (J:159285)