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A targeting vector was assembled by Red/ET recombination in which cre recombinase was driven by the Sykb regulatory elements. An FRT-flanked Pgk-neomycin selection cassette was included in the construct. The construct was electroporated into R1 ES cells. Correctly recombined clones were injected into C57BL/6 blastocysts to generate chimeras. (J:159112)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
