A loxP and FRT flanked neo cassette was inserted 5' and a third loxP site was inserted 3' of the coding sequence via homologous recombination. The selection cassette was removed by flp mediated recombination leaving the entire coding sequence floxed. (J:157841)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count