An frt flanked neo cassette and loxP site were inserted upstream of exon 1 and a second loxP site was inserted downstream of exon 1 vi homologous recombination. The neo cassette was then removed by Flp mediated recombination. (J:158898)
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An frt flanked neo cassette and loxP site were inserted upstream of exon 1 and a second loxP site was inserted downstream of exon 1 vi homologous recombination. The neo cassette was then removed by Flp mediated recombination. (J:158898)