Two loxP sites were inserted to flank exon 3 of the gene, followed by an SA-IRES-lacZ-neo-SVpA cassette and a third loxP site after the cassette in intron 3. The SA-IRES-lacZ-neo-SVpA cassette was deleted by Cre mediated recombination. (J:158671)
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Two loxP sites were inserted to flank exon 3 of the gene, followed by an SA-IRES-lacZ-neo-SVpA cassette and a third loxP site after the cassette in intron 3. The SA-IRES-lacZ-neo-SVpA cassette was deleted by Cre mediated recombination. (J:158671)