Pag1^tm1.1Tara

A floxed neomycin resistance cassette was inserted in intron 1 and a third loxP site was placed in intron 2 just downstream of exon 2. The selection cassette was then removed from properly targeted ES cells by transient cre expression. Homozygous mice were crossed to Tg(EIIa-cre)C5379Lmgd mice to delete exon 2. Western blot of protein from thymocytes and splenocytes of mice homozygous for the exon 2 deletion lacked expression of native protein and trucated forms. (J:159011)