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The coding sequence for cre recombinase, followed by an IRES and the DsRed2 sequence was placed downstream of a 3kb cholecystokinin (CCK) promoter. The construct was linearized, purified, and microinjected into the pronuclei of one-cell C57BL/6 embryos. Lines A, B, and C were established and analyzed. None of the three lines showed any observable DsRed fluorescence. Line C showed high levels of cre expression in the cortex, in a manner qualitatively similar to observed patterns of endogenous Cck mRNA expression, but showed almost no cre-mediated LacZ expression in any subcortical regions. (J:154361)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
