Exon 2 was flanked by loxP sites and a FRT flanked neo marker was inserted in intron 2 by homologous recombination. The selection cassette was deleted by Flpe recombinase. (J:157532)
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Exon 2 was flanked by loxP sites and a FRT flanked neo marker was inserted in intron 2 by homologous recombination. The selection cassette was deleted by Flpe recombinase. (J:157532)