The single coding exon 1 was flanked by loxP sites and an FRT flanked neo was inserted within intron 1 by homologous recombination. Flp mediated recombination removed the selection cassette. (J:156540)
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The single coding exon 1 was flanked by loxP sites and an FRT flanked neo was inserted within intron 1 by homologous recombination. Flp mediated recombination removed the selection cassette. (J:156540)