Gt(ROSA)26Sor^{tm1.1(CMV-luc,-ALPP)Cklr}

A dual-modality bioreporter was targeted to the Rosa26 locus in intron 1. In addition to the native Rosa26 promoter, the CMV immediate-early promoter/enhancer and the SV40 late viral protein gene 16S/19S splice donor and acceptor (sd/sa) signal sites were added to strengthen reporter gene activity. To minimize leakage, a stop cassette consisting of 6 tandem copies of the SV40 viral early and late polyadenylation signal sequences [(pA)6] were inserted downstream of the promoter elements, flanked by LoxP sites. The stop cassette is followed by firefly luciferase, a human IRES, and human placental secreted alkaline phosphatase. An FRT-flanked neo selection followed the hPLAP gene and was removed by breeding to FLPe transgenic mice. (J:157754)