The locus was floxed and an FRT flanked neo cassette was inserted centromeric to the locus by homologous recombination. The selection cassette was removed by flp mediated recombination and the locus removed by cre mediated recombination. (J:156946)
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The locus was floxed and an FRT flanked neo cassette was inserted centromeric to the locus by homologous recombination. The selection cassette was removed by flp mediated recombination and the locus removed by cre mediated recombination. (J:156946)