Kitl^tm3.1Pbes

A neomycin resistance cassette replaced exon 9, which encodes the C-terminal tail sequence. Splicing resulted in addition of 23 extraneous amino acids following exon 8 sequence. The absence of exon 9 was confirmed by sequencing. The floxed neomycin cassette was excised in vivo by Cre mediated recombination. Western blot analysis using extracts from the cerebellum showed that the total protein expression levels were similar to wild-type mice. ELISA showed a two-fold reduction of soluble protein levels in the plasma. (J:157170)