Tg(HLA-DRA,HLA-DRB1*0401)39-2Kito

A chimeric MHC class II complex was created by fusing the human peptide antigen binding domains of class II alpha and beta chains (HLA-DRA and HLA-DRBI*0401) to the remaining domains of murine class II alpha or beta chains (H2-Ea and H2-Eb1 genes that make up mouse I-Ed). The chimeric gene constructs were manipulated by replacing exon 2 of IEd-alpha or IEd-beta genomic DNA with gene fragments containing exon 2 of HLA-DRA or HLA-DRB1*0401 respectively. The native murine promoters were left intact. These transgene constructs were co-injected into blastocytes. Flow cytometry analysis indicates that B220+ cells co-express the chimeric MHC class II molecules. Line 39-2 is a representative of several lines generated. The chosen human alleles represent human serotype HLA-DR4. (J:109908)