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An EGFP fused to a humanized cre (hCre) sequence and preceded by an internal ribosomal entry site (IRES) was inserted into the 3' UTR of the Lyve1 locus by homologous recombination in E14 ES cells. This construct was followed by an FRT-flanked neo selection cassette. Three successfully targeted clones were injected into C57BL/6 blastocysts and chimeras derived from clone 9 were bred to get germline transmission. The neo cassette was deleted in the germline with Flp recombinase. Selective GFP expression in nuclei of Lyve1 +ve cells is detected by antibody staining. (J:156826)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
