A loxP site was inserted upstream of exon 3 and a floxed and frt flanked neo cassette was inserted downstream of exon 4. Flp mediated recombination removed the neo cassette leaving exons 3 and 4 floxed. (J:156027)
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A loxP site was inserted upstream of exon 3 and a floxed and frt flanked neo cassette was inserted downstream of exon 4. Flp mediated recombination removed the neo cassette leaving exons 3 and 4 floxed. (J:156027)