Tg(Gjb6-cre/ERT2)53-33Fwp

The creERT2 expression cassette, preceded by a rabbit beta-globin DNA encompassing intron 2 (beta-globin intervening sequences [bGIVS]) and followed by the SV40 polyA signal and a PGK/Tn5-driven neomycin resistance cassette flanked by FRT sites (neo) was inserted by homologous recombination at the transcription initiation site of the Gjb6 gene located in BAC RP24-368M7. The linearized vector was injected into pronuclei of fertilized FVB/N eggs. Line 53-33 displayed high quantities of CreERT2 transcripts in several brain regions including cortex, cerebellum, hippocampus, mesencephalon, and medulla oblongata. CreERT2 and Cx30 mRNA were also present in the skin. (J:156087)